Journal: Drug Design, Development and Therapy

Article Title: Baolier Capsule’s Secret Weapon: Piperine Boosts Cholesterol Excretion to Combat Atherosclerosis

doi: 10.2147/DDDT.S499598

Figure Lengend Snippet: Key Resources Table

Article Snippet: LDL-C assay kit , Elabscience , E-BC-K205-M.

Techniques: Control, shRNA, Sequencing, Staining, Fluorescence, Microscopy, AST Assay, Phospholipid Assay, Mass Spectrometry

Journal: Microbial Cell Factories

Article Title: Type 2 diabetes induced microbiome dysbiosis is associated with therapy resistance in pancreatic adenocarcinoma

doi: 10.1186/s12934-020-01330-3

Figure Lengend Snippet: T2D contributes to therapy resistance in PDAC. Schematic diagram of the experimental setup. Arrows indicate Streptozotocin (STZ) administration and fecal matter collection. C1, C2, C3, C4 refer to control groups that do not receive STZ or high fat diet D1, D2, D3, D4 refer to animals receiving STZ and put on high fat diet. C4 and D4 groups were further split into C4′ and D4′ to indicate groups that received Gem/Pac therapy ( a ). Blood glucose ( b ) and blood cholesterol ( c ) was measured at the end of 1 month to validate hyperglycemia and T2D onset in animals. After tumor implantation and 1 month of Gem/Pac, T2D group animals did not show any significant change in tumor volume ( e ) or weight ( f ), while the control tumors responded to the standard of care. *Indicates p value = 0.05

Article Snippet: Measurement of total cholesterol from mice serum were performed using cholesterol assay kit (Abcam) according to the manufacturer’s instructions.

Techniques: Control, Tumor Implantation

Journal: The FASEB Journal

Article Title: Intralipid Infusion to Fetal Sheep ( Ovis aries ) Promotes Differentiation and Lipid Accumulation of Adipose Tissues

doi: 10.1096/fj.202502000R

Figure Lengend Snippet: Effect of fetal Intralipid infusion on serum metabolic parameters. (A) Serum glucose concentrations. (B) Serum triglyceride (TG) concentrations. (C) Serum total cholesterol concentrations. (D) Serum low‐density lipoprotein cholesterol (LDL‐C) concentrations. (E) Serum high‐density cholesterol (HDL‐C) concentration. (Control group: N = 2F, 6M; Intralipid‐treated group: N = 6F, 3M; data are presented as mean ± SD; * p < 0.05, ** p < 0.01).

Article Snippet: Measurement of serum biochemical parameters, including triglycerides (TG), high‐density lipoprotein cholesterol (HDL‐C), and low‐density lipoprotein cholesterol (LDL‐C), was performed according to the manufacturers' instructions using commercially available assay kits (Novus Biologicals, Centennial, CO, USA; catalog numbers NBP3‐24542, NBP3‐25823, and NBP3‐25881, respectively).

Techniques: Concentration Assay, Control

Journal: NPJ biofilms and microbiomes

Article Title: Alleviation of Limosilactobacillus reuteri in polycystic ovary syndrome protects against circadian dysrhythmia-induced dyslipidemia via capric acid and GALR1 signaling.

doi: 10.1038/s41522-023-00415-2

Figure Lengend Snippet: Fig. 1 L. reuteri supplementation ameliorates dyslipidemia in constant darkness rats. a Timeline depicting the treatments of darkness and L. reuteri in different groups of the L. reuteri-treated rat model. b Representative estrous cycles. M, metestrus; D, diestrus; P, proestrus; E, estrus. c Quantitative analysis of estrous cycles. d Representative hematoxylin and eosin staining of ovaries. Asterisk stands for corpus luteum. Scale bar: 200 μm. e–g Serum concentrations of LH/FSH ratio (e), testosterone (f), and SHBG (g) detected by ELISA. h Body weight changes. i Representative Oil Red O staining of livers. Scale bar: 50 μm and 25 μm. j Representative images of liver ultrastructure detected by transmission electron microscope. Scale bar: 40 μm and 1 μm. k Hepatic contents of TG, CHOL, HDL-C, LDL-C, and NEFA detected by ELISA. l Serum concentrations of TG, CHOL, HDL-C, LDL-C, and NEFA detected by ELISA. Statistical analysis was performed with one-way ANOVA followed by Newman–Keuls multiple comparison test. n = 8 per group. Data present means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: The concentrations of TG, total CHOL, HDL-C, LDL-C, and NEFA in rat sera and liver tissues were detected using Triglyceride Quantification Assay Kit (#ab65336, Abcam), HDL and LDL/VLDL Cholesterol Assay Kit (#ab65390, Abcam), Rat LDL-C ELISA Kit (#CSB-E16561r, Cusabio, Wuhan, China), and Free Fatty Acid Quantification Assay Kit (#ab65341, Abcam), respectively.

Techniques: Staining, Enzyme-linked Immunosorbent Assay, Transmission Assay, Microscopy, Comparison

Journal: NPJ biofilms and microbiomes

Article Title: Alleviation of Limosilactobacillus reuteri in polycystic ovary syndrome protects against circadian dysrhythmia-induced dyslipidemia via capric acid and GALR1 signaling.

doi: 10.1038/s41522-023-00415-2

Figure Lengend Snippet: Fig. 2 Liver transcriptome analysis of L. reuteri-treated darkness rats. Heatmaps displaying 36 highly expressed genes (a) and 76 lowly expressed genes (b) in the liver of darkness rats compared with control and DL.reuteri rats through DEG analysis using Ballgown software (| fold change | > 0.6 in the log2 ratio value, raw P < 0.05). c Top terms from GO analysis (above) and terms from KEGG analysis (below) of the 112 differential genes in the DEG analysis. The P values of GO and KEGG analyses were determined on the DAVID website. d Visualization of the top GO and KEGG terms related with lipid metabolism and circadian rhythm in the DEG analysis. e Spearman rank correlations between module eigengenes (ME) and clinical biochemical index in the WGCNA analysis (|ρ | > 0.3, *P < 0.05). f Visualization of the top GO and KEGG terms related with lipid metabolism and circadian rhythm of purple module genes (102) in the WGCNA analysis. Circle, genes; Square, KEGG terms; Hexagon, GO terms. The bigger the square or hexagon, the more genes involved. g Crosstalk among different groups of genes identified the possible target genes taking essential roles in the lipid metabolism of L. reuteri-treated darkness rats. h mRNA abundances of Galr1, Galr2, Nr1d1, Nr1d2, Insig2, Srebf1, Lxra, and Rxra in rat liver detected by qPCR. β-Actin was used as a loading control for qPCR analyses. i Serum galanin concentration detected by ELISA. Statistical analysis (h, i) was performed with one-way ANOVA followed by Newman–Keuls multiple comparison test. n = 8 per group. Data present means ± SEM. *P < 0.05, **P < 0.01.

Article Snippet: The concentrations of TG, total CHOL, HDL-C, LDL-C, and NEFA in rat sera and liver tissues were detected using Triglyceride Quantification Assay Kit (#ab65336, Abcam), HDL and LDL/VLDL Cholesterol Assay Kit (#ab65390, Abcam), Rat LDL-C ELISA Kit (#CSB-E16561r, Cusabio, Wuhan, China), and Free Fatty Acid Quantification Assay Kit (#ab65341, Abcam), respectively.

Techniques: Control, Software, Concentration Assay, Enzyme-linked Immunosorbent Assay, Comparison